Determination of Amprolium Residues in Edible Tissues and Eggs of Poultry by Solid Phase Extraction-high Performance Liquid Chromatography
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Abstract:
An analytical method based on solid phase extraction-high performance liquid chromatography was established for the determination of amprolium residues in the edible tissues (muscle, liver, kidney, and fat) and eggs of chicken, ducks, and geese. First, amprolium in the sample was extracted by trichloroacetic acid-acetonitrile solution and purified by the solid-phase extraction column. A hydrophilic C18 chromatographic column was used; methanol, acetonitrile, and 6 mmol/L sodium 1-heptanesulfonate solution were used as the mobile phase for isocratic elution, with a detection wavelength of 268 nm. Amprolium exhibited an optimum linear relationship in the range of 0.1~10 μg/mL (r2=0.999). The limit of detection and limit of quantification were 150 μg/kg and 250 μg/kg, respectively, for amprolium in chicken, ducks, and geese muscle, whereas the limit of detection and limit of quantification of amprolium in the liver, kidney, fat, and eggs of chicken, ducks, and geese were 250 μg/kg and 500 μg/kg, respectively. When the addition levels in the blank tissues had a limit of quantification 0.5, 1, and 2 times the maximum residue limit, the average recoveries of amprolium ranged from 74.65%~96.24% and the intra-day and inter-day relative standard deviations were less than 15%. This method has high accuracy and precision, and is suitable for determining amprolium residues in the edible tissues and eggs of poultry, providing a feasible method for ensuring food quality control in routine applications.
