Preparation of Bifidobacterium adolescentis Microcapsules by Endogenous Emulsification
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
To improve the survival rate of Bifidobacterium adolescentis and its resistance to unfavorable environments, Bifidobacterium adolescentis microcapsules were constructed via endogenous emulsification with sodium alginate and whey proteins as composite wall materials. Simulated artificial gastrointestinal tests and storage stability tests were performed, and, using efficiency as an evaluation index, the effects of sodium alginate addition, the water-to-oil volume ratio, Tween 80 addition, stirring speed, and emulsification time were investigated based on a single factor design. Analysis and optimization of sodium alginate addition, Tween 80 concentration, and stirring speed were performed using response surface methodology; the optimal conditions for the preparation of Bifidobacterium adolescentis microcapsules were 2% sodium alginate, 0.6 mL Tween 80, and a stirring speed of 430 r/min, under which an encapsulation rate of 83.05% was obtained. Bifidobacterium adolescentis microcapsules were then placed in simulated artificial gastric and intestinal juices for 180 minutes to investigate bacterial survival, under which rates of 59.89% and 66.45% were obtained, respectively. Storage was then investigated, and optimum results of 56.83 % survival were obtained for microcapsules retained at 4 ℃ over 42 days. The results demonstrated good acid resistance and enteric solubility for Bifidobacterium adolescentis microcapsules produced by endogenous emulsification and indicated that capsules are stable when stored at 4 ℃.
