Synergistic Healing of Skin Injuries by Tilapia Skin Antioxidant Peptide and Vitamin C
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Abstract:
Caenorhabditis elegans (C. elegans) was used to investigate the impact of combining tilapia skin antioxidant peptide (TSAP) with vitamin C (Vc) for healing skin injuries. The survival rate, reactive oxygen species (ROS) content, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and catalase (CAT) activity of C. elegans were determined and the synergistic healing mechanism of TSAP and Vc was analyzed by molecular docking. The healing and survival rates of C. elegans in the treated groups (either TSAP alone or TSAP and Vc) were significantly higher than those in the control (P<0.05). The healing rate after 6 hours (21.84%) and survival rate after 144 hours (43.33%) obtained for C. elegans fed with TSAP and Vc were significantly higher than those of C. elegans fed with TSAP or Vc alone (healing rate of 14.11% and survival rate of 20.00% for TSAP-treated nematodes, P<0.05). Compared with the control, the intracellular ROS (34.28%) and MDA contents (44.47%) of C. elegans fed with TSAP and Vc decreased significantly (P<0.05), whereas the SOD (45.24%) and CAT activities (106.57%) significantly increased (P<0.05). Molecular docking analysis revealed that hydrogen bonds between Gly3, Arg8, and enediol enhance the synergistic healing effect. Conclusively, it is revealed that combining TSAP with Vc has a synergistic effect in terms of oxidative stress level regulation and healing activity in C. elegans.
