Ameliorating Effect of Goat Milk Casein Hydrolysates on Senescence of Caenorhabditis elegans
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Abstract:
In this study, the anti-aging and antioxidant activities of goat milk casein hydrolysates (GMCH) were studied in vitro and in vivo using a Caenorhabditis elegans model. The anti-aging effect of GMCH was studied by comparing the lifespan, exercise ability, lipofuscin level and other indicators of Caenorhabditis elegans between the GMCH experimental group and the E. coli OP50 control group. The in vitro and in vivo antioxidant activities of GMCH were evaluated by the free radical scavenging experiment sand the activities of antioxidant enzymes in C. elegans. At the same time, the lifespan of C. elegans under hydrogen peroxide, paraquat and heat stress conditions were determined. The results showed that compared with the control group, the average lifespan of the nematode fed with 0.1 mg/mL goat casein hydrolysate was prolonged by 9.33%, and the lipofuscin level in the body decreased by 65.00%. Meanwhile, GMCH had no negative impact on the reproductive and motor ability of the nematode, and had a certain protective effect on oxidative stress and heat stress of the nematode. GMCH had a certain in vitro antioxidant capacity and could improve the activities of antioxidant enzymes in vivo. Compared with the control group, the SOD activity of the experimental group increased by 43.30%, the CAT activity increased by 124.40%, the GSH activity increased by 176.90%, and the MDA content decreased by 78.70%. In summary, GMCH can prolong the lifespan of nematodes, reduce lipofuscin accumulation in nematodes, improve the resistance and the activities of antioxidant enzyme inside the body of nematodes under stress conditions, thereby having a good anti-aging activity.
