High-throughput Qualitative Screening of 221 Veterinary Drug Residues in Livestock and Poultry Meat by Liquid Chromatography-tandem Mass Spectrometry
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Abstract:
A high-throughput qualitative screening method for 221 veterinary drug residues in livestock and poultry meat based on liquid chromatography-tandem mass spectrometry was established. Livestock and poultry meat samples were extracted with 1% formic acid-acetonitrile, and the extract was dried under nitrogen in a water bath at 40 ℃ and reconstituted in 30% methanol-0.1% formic acid aqueous solution for determination. Gradient elution was carried out with methanol and 5 mmol/L ammonium acetate (containing 0.1% formic acid) as the mobile phase, with the flow rate being 0.3 mL/min, the injection volume being 2 μL, the column temperature being kept at 40 ℃, and C18 chromatographic column being used for separation. Multiple reaction monitoring (MRM) was performed simultaneously in positive and negative ion mode, and the compound was qualitatively analyzed based on retention time and ion abundance ratio. The peak shape of veterinary drug compounds was sharp and symmetrical. The detection limit of this method for pork, beef, mutton, chicken and duck matrices was between 0.1 μg/kg and 20 μg/kg, which can meet the basic requirements for screening. This method is simple and rapid, with high specificity no obvious interference. The method can realize the processing and screening of large quantities of samples in a short time period. It has been applied to the detection of actual samples with high detection rate and strong qualitative ability, and can be extended the application to the qualitative screening and detection for other animal-derived edible agricultural product matrices.
