Activity Evaluation of Polysaccharides from 10 Types of Edible Fungi Based using PCA and Entropy-weighted TOPSIS Method
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Abstract:
Ten common types of edible fungi with excellent functions were studied, and their major active polysaccharide was extracted. In vitro antioxidant activity and blood lipid-lowering function were used as evaluation indicators to comprehensively assess the functional properties of polysaccharides in edible fungus. Polysaccharides were extracted via aqueous extraction and alcohol precipitation, and their in vitro antioxidant and blood lipid-lowering activities were compared based on seven indicators. Principal component analysis (PCA) combined with orthogonal partial least squares discriminant analysis (OPLS-DA) and entropy-weighted technique for order preference by similarity to ideal solution (TOPSIS) method were applied to establish functional evaluation models. The quality of polysaccharides in edible fungi was comprehensively evaluated and sorted, and the samples were classified using cluster analysis. Total sugar content of the 10 edible fungi ranged from 48.61% to 64.43%. Different polysaccharides in edible fungi played diverse active functions. Two principal components were extracted by PCA, with their cumulative contribution rate reaching 86.05%. The contribution rate of each principal component indicated that the scavenging rates of superoxide anion radicals (•O2-), hydroxyl radicals (•OH), DPPH• radicals were the key influencing factors. The entropy-weighted TOPSIS method showed that the similarity of polysaccharides in the ten types of edible fungi was 0.194~0.882. The comprehensive score showed that the similarity of Lentinula was the highest at 0.882, and its functional properties were the best. Cluster analysis revealed that the polysaccharides of the edible fungi could be divided into four taxa based on their functional characteristics. In conclusion, a functional evaluation system was constructed to screen the polysaccharides of edible fungi with good functions. This study provides theoretical support for future in-depth research and lays a theoretical foundation for the development of functional products.
