Inhibitory Effect of Moringin from Moringa oleifera Seeds on Listeria monocytogenes
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Abstract:
To investigate the inhibitory effect of moringin on Listeria monocytogenes, the antibacterial activity of moringin against L. monocytogenes was evaluated by the Oxford cup method, the double dilution method, and the growth curve. The effects of moringin on cell membrane permeability of L. monocytogenes were explored through determining nucleic acid leakage and protein leakage, total extracellular sugar, and PI staining. The effect of moringin on L. monocytogenes biofilm was evaluated by crystal violet staining, bacterial surface hydrophobicity, bacterial mobility, and scanning electron microscopy. The effect of moringin on apoptosis of L. monocytogenes was analyzed based on intracellular Ca2+ content and apoptosis rate. The results showed that moringin had a strong antibacterial activity against L. monocytogenes, and the minimum inhibitory concentration (MIC) was 400 μmol/L. The growth of L. monocytogenes was significantly inhibited after the treatment with 1 MIC moringin, with the cell membrane damage rate reaching 50.20% while the permeability significantly increasing (P<0.05). The leakages of macromolecular substances such as nucleic acids, proteins, and carbohydrates were 2.59, 2.38, and 1.34 times that of the control group, respectively (P<0.05). Compared with the control group, the biofilm formation, bacterial surface hydrophobicity, motility, and adhesion decreased significantly (by 58.10%, 38.00% and 68.19%, respectively) (P<0.05). The intracellular Ca2+ content increased significantly (by 3.83 times; P<0.01), and the apoptosis rate reached 54.40%. Moringin may play an antibacterial rolethroughinhibiting biofilm, changing cell membrane permeability and promoting apoptosis. This study lays a theoretical foundation for the application of moringin in food preservation.
