Alliin Improves Lipid Accumulation in HepG2 Cells by Activating Autophagy
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Abstract:
The effect of alliin on palmitic acid-induced lipid accumulation in HepG2 cells was studied, and the relationship between lipid metabolism and autophagy was also explored in the article. The cell activity was measured by CCK8 method, the intracellular lipid accumulation was observed by Nile red staining, the contents of triglyceride and total cholesterol were detected by ELISA kits, and the content of autophagy marker protein was determined by Western blot. The results showed that compared with the blank group, the contents of total cholesterol and triglyceride in HepG2 cells in the positive control group increased significantly, about 1.69-times and 1.36-times respectively; Under the action of alliin in the concentration range of 40~160 µmol/L, the cell lipid droplets decreased, and the contents of total cholesterol and triglyceride decreased significantly in a dose-dependent manner. Under the action of allicin in the concentration range of 160 µmol/L, the contents of total cholesterol and triglyceride decreased significantly, 37.52% and 33.33% respectively. Meanwhile, alliin significantly increased the ratio of autophagy marker protein LC3-Ⅱ/LC3-Ⅰ in the concentration range of 80~160 µmol/L, increasing by 23.87% and 66.13% respectively. Therefore, alliin improved the lipid metabolism disorder in HepG2 cells induced by PA (palmitic acid), and may ameliorate the lipid accumulation by activating the activity of autophagy.
