Hypotensive, Hypouricemic, and Hypoglycemic Effect of Inactivated Bacterial Postbiotics in Vitro
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Abstract:
Postbiotics have various health benefits for the human body and have become a hot research topic in recent years. A commercially available, inactivated bacteriophage-type postbiotic product has been developed and prepared using soybean protein isolate as the main raw material. The scarcity of reports on such postbiotic products is the motivation for this study. To understand the biological activity of this postbiotic product, in this study, rat thoracic vascular smooth muscle cell (VSMC), human renal tubular epithelial cell (HK-2), and mouse preadipocyte (3T3-L1) models were used to evaluate its in vitro hypotensive, hypouricemic, and hypoglycemic properties. The results showed that the postbiotic product could significantly inhibit the migration and oxidative stress of VSMC cells induced by angiotensin II (Ang Ⅱ) in a concentration-dependent manner, with a 12.44% reduction in migration rate at a concentration of 200 μg/mL, and the release of reactive oxygen species (ROS) being 86.51% of that of the control group. In the HK-2 cell model, the postbiotic significantly reduced the uric acid content of HK-2 cells, with a 30.02% reduction at a concentration of 700 μg/mL. In contrast, evaluation of hypoglycemic activity showed that the postbiotic was unable to promote glucose uptake by insulin-resistant (IR) 3T3-L1 cells. The results of this study showed that the postbiotic has considerable hypotensive and hypouricemic biological activity, thus providing a theoretical basis for the development of functional foods or medical and health products with hypotensive and hypouricemic effects. At the cellular level, the postbiotic does not exhibit hypoglycemic effects, which need to be verified in animal models.
