Comparison of Two Salmonella Detection Methods Based on Recombinase Aided Amplification Technology
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Abstract:
Two rapid detection methods for Salmonella in food were established and compared using recombinase-mediated amplification technology. The primers and probes were designed based on the fimY gene of Salmonella, and the amplified products were detected using the fluorescence method and lateral flow strip method, respectively. A recombinase-mediated isothermal nucleic acid amplification method was constructed to verify the specificity, sensitivity, and detection effect of the two methods on artificially contaminated samples. The two methods constructed in this study were operated at a constant temperature of 39 ℃, and the detection time was 30~40 min. These methods have good specificity and no cross-reaction with other common food-borne pathogens, such as Escherichia coli and Staphylococcus aureus. The sensitivity of the lateral flow test strip method reached 1 pg/μL, and the sensitivity of the fluorescence method was 10 pg/μL. The detection results of the artificially contaminated samples were consistent with the detection results of the traditional separation and culture method. Therefore, two rapid, specific and sensitive methods were established in this study for the detection of Salmonella, which provide a confirmed reference value and data to support the rapid screening of Salmonella contamination in food.
