Antioxidant and Antitumor Activity of Black Ginseng Polysaccharides
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Abstract:
In order to develop the application potential of black ginseng polysaccharides in the food and medicine industries, the physical and chemical properties of polysaccharides were determined using high performance liquid chromatography and other methods. The antioxidant and antitumor activities of polysaccharides were evaluated through in vitro antioxidative and tumor cell inhibition experiments. A single component, BGP-1 (molecular weight 1.80×106 u) was separated from black ginseng polysaccharide using a Sephadex G-200 column chromatography. The polysaccharides were found to be mainly composed of glucose, arabinose, and mannose, with a molar ratio of 70.52:17.35:5.63, and also contained a small amount of galactose and xylose. Ultraviolet spectrum scanning showed that the black ginseng polysaccharide had no obvious impurities, and Fourier infrared spectrum showed that it was a neutral polysaccharide with α-glycoside configuration. In the antioxidant experiment, polysaccharides from black ginseng showed a certain antioxidant activity in the range of 0.8~4.0 mg/mL, and all could achieve the effect of more than 62% under the same concentration of Vc. The MTT method and flow cytometry experiments confirmed that black ginseng polysaccharides inhibited the growth of MGC-803 gastric cancer cells, and the inhibition rate reached 85.30% at 4.0 mg/mL. The Western Blot results showed that 0.8~4.0 mg/mL black ginseng polysaccharide promoted cell apoptosis through the signal transduction of the mitochondrial pathway. In conclusion, black ginseng polysaccharides have antioxidant and antitumor activities, which can be used in the field of pharmaceutical preparations after research and development.
