The Intestinal Absorption Characteristics of Compound Safflower Seed Oil Investigated by the Everted Gut Sac Technique
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Abstract:
The intestinal absorption characteristics of compound safflower seed oil was investigated. Its intestinal absorption site, time and loading concentration were investigated by the everted gut sac technique. The contents of linoleic acid (LA) and α-linolenic acid (ALA) in the the collected intestinal fluid were determined by gas chromatography-mass spectrometry (GC-MS). The intestinal transport mechanism was analyzed through calculating the absorption transport parameters and absorption rate. In different intestinal segments, the main components LA and ALA were detected in both the duodenum (LA: 2.71 mg; ALA: 1.60 mg) and jejunum (LA: 1.89 mg; ALA: 1.43 mg). By calculating the permeation volume (M), absorption conversion rate (A) and residue percentage (R) at different sites, it was found that the M values of LA and ALA in the duodenum were higher than those for the jejunum, the A values were close to those for the jejunum, and the R values were lower than those for the jejunum. Therefore, the duodenum was the best absorption site among the intestinal segments. At the 120th min, the Pappi (apparent permeability coefficient) values of LA and ALA were higher than those of other time points, thus, the optimal absorption and transport time was 120 min. When the loading dose was 2.0 times, the concentrations of LA and ALA in the intestinal sac fluid were the highest (0.35 and 0.19 mg/mL, respectively), thus, the optimal loading dose was 200 mg/mL. The results showed that the intestinal absorption of the compound safflower seed oil may take place in a time- and concentration-dependent passive transport mode and via one-way transport in the duodenum.
