Simultaneous Quantitative Analysis of Plant Metabolites in Different Parts of Perilla (Perilla frutescens) by HPLC
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Abstract:
This study aims to establish an analytical method for the simultaneous detection of metabolites in different parts of Chinese Perilla frutescens. Identification of the types and concentrations of metabolites will provide a scientific basis for efficient utilization of P. frutescens compounds. The 19 plant metabolites in the leaves, seed shells and stems of Chinese perilla (Perilla frutescens) were analyzed by solvent extraction combined with high-performance liquid chromatography technique. Results indicated that the 19 compounds were completely separated within 55 minutes with good peak shape and separation, a limit of detection (0.05~0.40 mg/L), correlation coefficients (R2) over 0.997 6, recoveries of 61.65%~111.42%, and precision of 1.02%~17.20%. This method was used to analyze 12 kinds of perilla samples, and it was found that the contents of 19 compounds in different parts of perilla varied significantly in the range of 0.04~24.05 mg/g. Two phenolic acids and five flavonoids and flavonoid glycosides were detected in the 12 samples. Luteolin was detected in perilla shells and stalks, with the contents with the contents ranging between 0.05 and 0.18 mg/g; the decreasing order of compound content in perilla leaves was rosmarinic acid > scutellarin > apigenin-7-O-glucoside. In addition, two furan ring derivatives, HMF and furfural, were detected for the first time in the perilla shells of this study. The method is simple and efficient, and the instrumental requirement is low, thus, is suitable for quantitative determination of metabolites in Perilla samples and other types of plant samples.
