Isolation and Purification of Lycium Barbarum Polysaccharides and Analysis of its in Vitro Anti-proliferative and Anti-inflammatory Activities
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Abstract:
To investigate the effects of Lycium barbarum polysaccharides on the proliferation of Estradiol (E2) and progesterone (P) induced-mouse mammary gland epithelial cells (HC11) and LPS-induced inflammatory response in macrophages. Different components were obtained by water extraction, ethanol precipitation and column separation, and their molecular weights, and in vitro anti-proliferative and anti-inflammatory effects were preliminarily analyzed. The result showed that seven Lycium barbarum polysaccharides components were eluted out by DEAE-52-ion chromatography column and Sephadex G-50 gel column (GQ-01, GQ-02, GQ-03, GQ-04, GQ-05, GQ-06 and GQ-07, respectively), and their mean relative molecular weights were 113 ku, <1 ku, 367 ku, 613 ku, 81 ku, 110 ku, and <1 ku. Different Lycium barbarum polysaccharide concentrations (5, 25, 125, 250, 500, 1 000 μg/mL) were used to treat HC11 cells, and GQ-01, GQ-04 or Gq-06 at 250 μg/mL could significantly inhibit the proliferation of HC11 cells co-treated with E2 (25 μg/mL) and P (250 μg/mL) by 28.13%, 26.74% and 30.83%, respectively (P<0.01). GQ-01 and Gq-06 could regulate the G0/G1 and G2/M phase transition to affect the cell cycle distribution. Compared with the model group, Gq-06 could significantly inhibit the E2+P induced ERα level (decreased by 5.54%) (P<0.01). Compared with the model group, the production of NO in macrophages could be significantly inhibited by GQ-04 and GQ-06 (by 13.43% and 12.65%, respectively) (P<0.05). This research showed that GQ-06 may inhibit the excessive proliferation of mammary gland epithelial cells by affecting the cell cycle distribution and the level of ERα, and also suppress LPS-induced NO release to reduce the inflammatory response in RAW264.7 cells. This study will provide an important theoretical basis for further development of functional foods.
