Rapid Screening and Quantitative Analysis of 22 Illegally Added Compounds in Gut Health-improving Foods by High-resolution Mass Spectrometry
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Abstract:
A method for rapid screening and quantitative analysis of 22 kinds of illegally added compounds in health-promoting foods using ultra-performance liquid chromatography-quadrupole/electrostatic orbitrap high-resolution mass spectrometry was established. In this paper, the commonly marketed gut function-improving healthcare products (tablets and oral liquid matrices) were taken as the research objects. Samples were extracted with methanol by vortex oscillation and ultrasonic treatment, and separated on an Agilent ZORBAX Eclipse XDB-C18 column (2.1 mm×50 mm,1.8 μm) using 0.1% formic acid aqueous solution and methyl alcohole as the mobile phase for gradient elution. Mass spectrometry analyses adopted a positive ion scan and full MS/dd-MS2 mode, and a database was established based on the retention times of compounds, primary parent ions and secondary fragment ions information collected automatically for high-throughput qualitative screening and quantitative analyses with primary parent ions. The 22 kinds of chemical compounds were well separated within 6.5 minutes with the mass accuracy deviation less than 1.77×10-6. The results showed a good linear relationship for each target compound in the concentration range of 5 ng/mL~500 ng/mL (r2 >0.995). The limits of quantitation (LOQs) were 50 μg/kg. The recoveries were 82.11%~116.16% for tablet samples and 83.64%~117.21% for the oral liquid samples. This method is simple, rapid, sensitive and accurate, thus suitable for rapid screening and quantitative analysis of 22 kinds of illegally added compounds in gut function-improving healthcare products in tablet and oral liquid forms.
