Modeling and Optimization of Fermentation by Lipase MAS1-producing Recombinant Escherichia coli Based on Support Vector Machine
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Abstract:
In this study, the lipase MAS1 from marine actinomycetes (Streptomyces sp.) W007 was used as the research object, which was expressed in Escherichia coli BL21 (DE3) and associated fermentation conditions were optimized. First of all, the optimal induction time for the expression of lipase in recombinant Escherichia coli (E. coli) was determined by the single factor experiments to be the late logarithmic growth phase, with the optimal Isopropyl-β-D-thiogalactopyranoside (IPTG) concentration being 0.6 mmol/L, the optimal induction pH being 6.5, and the optimal induction temperature being 20 ℃. Then, the Box-Behnken test was designed by the R language rsm package, and was conducted in a 7 L fermentor to obtain the data. Finally, the optimal fermentation conditions for the expression of lipase MAS1 in the recombinant E. coli BL21(DE3) were calculated by support vector machine (SVM)-genetic algorithm (GA): the optimized IPTG concentration, induction temperature and induction pH were 0.65 mmol/L, 23 ℃ and 6.7, respectively, which led to the theoretical enzyme activity of lipase MAS1 as 2 276.99 U/mL. In the 7-L fermentor, the maximum enzyme activity of lipase MAS1 was 2 316.02 U/mL under the optimized fermentation conditions for culturing recombinant E. coli BL21(DE3). The above research results show that the SVM-GA exhibits good performance during the optimization of fermentation conditions for recombinant E. coli, which provides a research basis for the efficient preparation of lipase MAS1.
