Genome Structure and Capsid Protein Function of the Foodborne Norovirus GII.2[P2] Strain in China
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Abstract:
Foodborne norovirus is the leading microbial pathogens that causes food safety incidents worldwide and has abundant genetic diversity. In recent years, GII.2 has gradually become one of the main prevalent genotypes in China. In order to understand the variation characteristics of this food-borne norovirus GII.2, the GZ2015-L335 strain of GII.2[p2] isolated in Guangzhou in 2015 was used as the research objective, and the genome structure and capsid protein function of this strain were characterized. The full length of the gene of this strain was 7 496 bp. The results of online genotyping showed that it was GII.2[P2] genotype. The recombinant capsid protein VP1 was obtained by cloning the capsid protein VP1 gene and using the recombinant baculovirus expression system, then purified by cesium chloride density gradient centrifugation. The results of SDS-PAGE and Western blot showed that the molecular weight of the recombinant protein VP1 was about 58 ku. The transmission electron microscopy examinations showed that the VP1 could self-assemble into virus-like particles (VLP) with a diameter of approximately 30 nm. The results of ELISA showed that the VLP had high binding activity for the serum against GII.2[P2] capsid P particles. In addition, the receptor binding assay showed that the VLP exhibited broad binding affinities to both secretory salivary receptors (including A/B/O) and non-secretory salivary receptors. In summary, this study systematically analyzed the genome structure of the GII.2[P2] strain, and successfully prepared and characterized its virus-like particles. The obtained results would provide support for the development of highly effective antibody and novel detection technology for food-borne norovirus.
