Abstract:Cascading enzyme complexes can enhance cascade enzymatic activity through substrate channeling, which succeed in industrial process development, such as synthesis of pharmaceutical, cosmetic, and functional foods. The variety of reactions provided by enzyme has promoted biocatalysts been as a green economic sustainable production tool in modern synthetic chemistry. Nevertheless, co-immobilized cascading enzyme complexes are favorable to large-scale industrial manufacture. The advantages of using immobilization include not only improved cascade enzymatic activity but also enhanced enzyme stability and ease of recovery for reuse. Most noteworthily, characteristics of enzyme catalysis, immobilization methods, corresponding reaction kinetics are the key factors for activity and stability of enzyme complexes. As to sufficient coordination, the development in biology and materials science, such as the research and development of loading materials with nano characteristics and more stable properties, probably has provided for design of ideal multienzyme complexes devices. Based on different bonding methods and loading materials, recent advanced in bioreactions via co-immobilized multi-enzyme were described in this review.
