Abstract:Lactobacillus plantarum were isolated from Sichuan Paocai and screened for probiotics. The strains were separated using MRS agar medium, and then identified using Gram staining, biochemical identification, and molecular biology methods. The VITEK 2 Corynebacterium identification card (CBC) includes a total of 41 biochemical reactions and enables identification to the species level. Of the 20 strains of bacteria detected, 12 strains were identified as L. plantarum using the CBC cards. These strains were subjected to16S rRNA PCR amplification and sequencing, followed by homologous sequence alignment on the NCBI website. The phylogenetic tree constructed by the neighbor-joining method showed that all 12 strains were clustered with known L. plantarum sequences. Strain No. 6 was clustered with L. plantarum 3356. Strain numbers 4, 5, 14, 15, 23, and 38 had very high sequence similarity with L. plantarum NCU116, L. plantarum MBEL2169, and L. plantarum AN7, with no genetic distance. In conclusion, all 12 strains were identified as L. plantarum. CBC identification cards are an effective method for the identification of L. plantarum.