Alleviating Acute Alcohol Intoxication in Mice using Yinghong No.9 Black Tea Extracts to Regulate the TNF-α/NF-κB Signaling Pathway
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Abstract:
To study the effects and mechanisms of Yinghong No.9 black tea water-extract (YBTE) for alleviating acute alcohol intoxication (AAI), mice were subjected to one-time intragastric administration of 56° Baijiu (Chinese Liquor) to establish an AAI model. Subsequently, the time required to lose and regain the righting reflex of the mice were recorded. The alanine transaminase (ALT) and glutamic oxaloacetic transaminase (AST) activities in the serum and alcohol dehydrogenase (ADH) activity both in the serum and liver were measured. Moreover, the expression the nuclear factor kappa B (NF-κB), tumor necrosis factor-α (TNF-α), and interleukin-1 β (IL-1β) proteins in the liver were measured using western blot analyses. The results showed that sleep onset latency increased and sleep duration decreased for mice treated with various doses of YBTE compared with the model group. More specifically, the sleep onset latency for the high-dose YBTE-treated group increased to 40.89 min (p<0.01) and their sleep duration was 166.18 min (p<0.01). Moreover, the serum activities of ALT and AST were significantly lower and the ADH activity in the serum and liver were both significantly higher in the YBTE-treated groups than in the control group. In particular, the ALT and AST activities in the high-dose group decreased to 22.89 U/L (p<0.01) and 29.58 U/L (p<0.01), respectively, and the ADH activity in the serum and liver increased to 43.43 IU/mL (p<0.01) and 7.37 IU/mL (p<0.01), respectively. Furthermore, the expression of p-NF-κB p65, TNF-α, and IL-1β in the liver were significantly down regulated. In conclusion, AAI can be effectively relieved with YBTE by promoting ethanol metabolism and down regulating the TNF-α/NF-κB signaling pathway.
