Comparative Study on GB and AOAC Methods to Determine Primary B Vitamins in Milk Powder
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Abstract:
The equivalence between the national standard and official AOAC methods for determining vitamins B1, B2, B3 (niacin), and B6 in milk powder was analyzed by performing the F-test and significance test on the obtained measurements to explore the comparability and error sources between these methods. Furthermore, vitamin B2 results were compared and analyzed in detail by a two-way one-sided t-test (TOST). The results indicated no significant difference between the national standard and AOAC official methods in determining vitamins B1, B3, and B6. However, for vitamin B2, a difference was observed between the two methods in terms of their mechanisms. The equivalence analysis results showed that the two methods are not effectively equivalent. Addition of acid phosphatase to the milk powder matrix was found to help obtain comparable results.
