Antioxidant and Whitening Activities of Heat-transformed American Ginseng Stem-leaf Saponins
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Abstract:
The levels of less-polar ginsenosides in American ginseng stem-leaf saponins and the antioxidant and whitening activities of American ginseng stem-leaf saponins before and after heat transformation were studied. The results showed that the content of less-polar ginsenosides in American ginseng stem-leaf saponins increased from 0.07 mg/mg to 0.66 mg/mg during heat transformation. Moreover, when the concentration of American ginseng stem-leaf saponins before and after transformation was in the range of 0.1~5 mg/mL, the maximum of DPPH free radical scavenging rates were 16.65% and 38.28%, respectively, ABTS free radical scavenging rates were 8.20% and 41.94%, respectively, and the reducing capacity was 3.24 mg TEAC/g DW and 25.22 mg TEAC/g DW, respectively. In addition, the concentration of American ginseng stem-leaf saponins before and after thermal transformation was in the range of 0.025~0.1 mg/mL, the maximum inhibition rates of tyrosinase activity in B16 cells were 30.22% and 33.54%, respectively, and the maximum inhibition rates of melanin production in B16 cells were 17.27% and 29.33%, respectively. In conclusion, the content of less-polar ginsenosides in American ginseng stem-leaf saponins was increased, and the antioxidant and whitening activities of American ginseng stem-leaf saponins were enhanced after heat transformation treatment, which has great application value in the fields of beauty and medicine.