Regulation of Flaxseed Oil on Inflammatory Cell in Rats with Polycystic Ovary Syndrome
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Abstract:
The regulatory effects of alpha-linolenic acid (ALA)-rich flaxseed oil (FO) on myeloid-derived suppressor cells (MDSCs), regulatory T cells (Tregs), and macrophages (Mψs) in a rat model of letrozole-induced polycystic ovary syndrome (PCOS) were investigated. Changes in the estrous cycle were also monitored every day. After successful model establishment, the FO control group and FO-treated model group were administered FO via gavage feeding for 8 w, whereas the negative control group and model group were administered the same as well as Mψs, M1 Mψs and M2 Mψs in ovary of rats in diverse groups were respectively detected by flow cytometry; the expression of Mψs amount of normal saline. After 8 w of treatment, the percentages of MDSCs in the peripheral blood, spleen, and bone marrow, those of Tregs in the peripheral blood and spleen, and those of Mψs, M1 Mψs, and M2 Mψs in the ovaries of rats in all four groups were evaluated using flow cytometry. The expression of Mψs in the ovarian tissues was determined using immunofluorescence staining. According to the results, letrozole-induced PCOS was successfully established in the rats. Moreover, dietary FO treatment significantly increased the percentages of MDSCs in the peripheral blood, spleen, and bone marrow to 25.87%, 5.63%, and 28.36%, respectively. Additionally, the percentages of Tregs in the peripheral blood and spleen were increased to 2.06% and 1.07%, respectively (p<0.05). By contrast, the percentages of ovarian Mψs and M1 Mψs had declined to 13.51% and 2.27%, respectively, whereas the fluorescence intensity of ovarian Mψs had decreased to 1.59% (p<0.05). Our results indicate that dietary ALA-rich FO supplementation may ameliorate PCOS by recruiting MDSCs in the peripheral blood, spleen, and bone marrow; increasing Tregs in the peripheral blood and spleen; and suppressing Mψ proliferation and M1 Mψ polarization in the ovaries.
