This study aimed to evaluate the performance of the micro-biochemical identification kit for Yersinia enterocolitica developed in our laboratory (termed EasyID). Thirty six strains of Y. enterocolitica (including two standard strains and nineteen isolated strains of Y. enterocolitica; five isolated strains of Y. flexneri; five isolated strains of Y. intermedia; five isolated strains of Yersinia Klebsiella) were used to evaluate the three kinds of Y. enterocolitica identification reagent kits: EasyID, traditional liquid biochemical tube (HKM) and other domestic brands (termed KIT). The results showed that the identification results of the thirty six tested strains exhibited overall coincidence rates of 99.69% and 98.15%, respectively, for the “dry” identification kits, EasyID and KIT, compared with those of the traditional liquid identification kits. Compared with KIT, EasyID had a higher coincidence rate with HKM; the three identification kits, EasyID, HKM and KIT, had overall compliance rates of 99.37%, 99.07%, and 97.83%, respectively, compared with the standard requirements, with the EasyID having the highest conformity rate, followed by HKM then KIT. Conclusion: The reliability of EasyID Yersinia enterocolitica microbiochemical identification kit was superior to KIT and comparable to HKM. In terms of ease of use, EasyID involving one-step sampling technique was superior to HKM and KIT.
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