GC-MS Analysis of the Changes in Volatile Oils before and after Compounding of Perilla Leaf and Tangerine Peel
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Abstract:
Steam distillation was performed to extract the volatile oils of Perilla leaf, tangerine peel, and the compound of Perilla leaf and tangerine peel (hereinafter referred to as the compound) to investigate changes in volatile oils before and after the compounding of Perilla leaf and tangerine peel. The volatile oils were analyzed by gas chromatography-mass spectrometry to compare the differences in their composition and content. A total of 20, 22, and 31 components were identified from the volatile oils of Perilla leaf, tangerine peel, and the compound, respectively, and the total mass fractions of the detected substances were 97.77%, 99.76%, and 97.94%, respectively. Four components in the volatile oils of Perilla leaf (menthol, β-bourbonene, trans-nerolidol, and humulene epoxide II) were not detected in the volatile oils of the compound, whereas the content of β-copaene increased significantly after compounding. Five components in the volatile oils of tangerine peel (2-carene, p-cymene, isoterpinolene, 4-terpenol, and germacrene) were not detected in the volatile oils of the compound, and the content of cyperenone increased significantly after compounding. Six additional components were detected in the volatile oils of the compound (4-carene, o-isopropyl benzene, decanal, γ-elemene, neryl acetate, and nerolidyl acetate). The results showed that there were significant changes in the components of volatile oils before and after compounding of Perilla leaves and tangerine peel. This study provides new insights for the further development of Perilla leaf and tangerine peel.