Analysis of Chemical Constituents in Different Parts of Hellgrammites Based on UHPLC-Q-TOF-MS/MS
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Abstract:
UHPLC-Q-TOF-MS/MS was used to analyze the chemical constituents of the different parts of hellgrammites such as head, thorax and abdomen. The differential material contents were detected. The distribution patterns of the chemical constituents of hellgrammites was discussed. The ultra high performance liquid chromatography conditions were: Waters acquity BEH amide column, 100 mm × 2.1 mm, 1.7 μm; mobile phase, 25 mmol/L aqueous ammonium acetate solution and acetonitrile in gradient mode, flow rate 0.2 mL/min; column temperature, 30 ℃. Mass spectrometry conditions were: Triple TOF 6600 high resolution tandem mass spectrometry, electrospray ionization (ESI) ion source, and data collection in positive and negative mode. A total of 25 compounds were identified in hellgrammites by means of positive and negative ion mass spectrometry information and element composition analysis. Among which, 19 compounds in the head, 21 compounds in the thorax and 19 compounds in the abdomen (including nine amino acids, six organic acids, five nucleosides and five other compounds), with 19 compounds being reported for the first time in hellgrammites. The contents of the differential constituents in different parts of hellgrammites were determined by HPLC. The results showed that there were significant differences in the contents of the differential components in the head, thorax and abdomen (p<0.05). This research showed an analytical method with high sensitivity, and high degree of separation for rapid analysis of the chemical composition of hellgrammites and identification of the differential compounds in different parts, which provides a basis for fundamental research of hellgrammites’ substances and the selection of their usable parts.
