Protective Effect of Coreopsis tinctoria Nutt. Ethanol Extract against Acrylamide-induced Liver Injury
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Abstract:
The ethanol extract of Coreopsis tinctoria Nutt. was applied to human hepatocellular carcinoma HepG2 cells and mouse liver to evaluate its protective effect against acrylamide (ACR)-induced injury. Cell survival rate was used to evaluate the protective effect of C. tinctoria ethanol extract against HepG2 cell injury, whereas body weight change, liver function, and liver pathological sections were used to evaluate the protective effect of C. tinctoria ethanol extract against mouse liver injury. Antioxidant indices were also determined to evaluate the underlying protective mechanisms. The results indicated that after preincubation with C. tinctoria ethanol extract (2.00 mg/mL), the survival rate of ACR-injured HepG2 cells increased by 12.81%; intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) decreased by 57.67% and 4.68 nmol/mg, respectively; and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels increased by 4.68, 10.48, and 13.81 U/mg, respectively. The weight gain of mice in the low-dose (0.25 g/kg), medium-dose (0.25 g/kg), and high-dose (0.25g/kg) groups of C. tinctoria ethanol extract were 2.2~2.5 times that of the ACR group. In the high-dose group (1.0 g/kg), serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) decreased by 27.76 and 46.79 U/L, respectively; MDA decreased by 1.86 nmol/mg, whereas SOD and GSH-Px increased by 56.73 and 330.44 U/mg, respectively. Hematoxylin and eosin staining results of liver tissue showed that the hepatic lobule structure and hepatocyte morphology of mice had improved to varying degrees with low, medium, and high doses of C. tinctoria ethanol extract, with significant alleviation of the injury to liver tissue. In conclusion, C. tinctoria ethanol extract could prevent the ACR-induced injury of HepG2 cells and mouse liver tissue, and the underlying mechanism was related to its antioxidant capacity.
