Regulation of Vomit Toxin on Proliferation and Apoptosis Inducing Protein Expression of HK2 Cells
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Abstract:
This paper investigated the effects of vomit toxin on inhibiting the proliferation and inducing the expression of apoptotic protein in HK2 cells. After different concentrations of vomit toxin acted on HK2 cells, the inhibitory effect of vomit toxin on cell proliferation was detected by MTT method, the changes of cell morphology were observed by fluorescence microscope, the changes of apoptosis rate were detected by flow cytometry, and the expression levels of apoptotic proteins Bax, Bcl-2, NF-KB, caspase-2, 3, 6, 8 and 9 were detected by Western blotting. The results showed that vomit toxin could significantly inhibit the proliferation of HK2 cells in a dose-response relationship, at the concentration of 15, 30, 40, 60 and 80 μmol/L, the inhibitory rates of vomit toxin on HK2 cells were 61.82%, 53.64%, 42.43%, 38.66% and 36.54%, respectively; The survival rates of HK2 cells treated with 30 μmol/L of vomit toxin for 0, 12, 24, 48, 72 and 96 hours were 99.84%, 80.51%, 60.25%, 52.22%, 62.50% and 71.34% respectively. The apoptosis rates of cells treated with 15, 40 and 80 μmol/L of vomit toxin for 24 hours were 2.30%, 30.20% and 53.50% respectively. The Western bloting results showed that vomit toxin upregulated Bax, Caspase-2, 3, 6, 8, 9 protein expression and inhibited the protein expression of bcl-2 and NF-KB. The results showed that vomit toxin significantly inhibited the proliferation of HK2 cells, caused apoptosis and regulated the expression of apoptotic protein.
