Screening of Hypolipidemic Lacticaseibacillus rhamnosus and Exploration of Its Effect on High-fat Hamster Models
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Abstract:
Screening was performed for a strain of Lactobacillus with strong cholesterol reduction ability from experimental strains isolated from the intestinal tract of healthy infants. The cholesterol reduction ability of the strains was measured using the o-phthalaldehyde colorimetric method, whereas triglyceride reduction ability was measured using the GPO-PAP method. The tolerance of the strains to acids and bile salts was also measured. Strains with strong cholesterol and triglyceride reduction functions were selected and confirmed using 16S rDNA. The hypolipidemic ability was studied by establishing a hyperlipidemic hamster model. Results showed that Lacticaseibacillus rhamnosus HCS01-013 had strong cholesterol and triglyceride reduction ability, with cholesterol degradation rate reaching 35.40% and triglyceride degradation rate reaching 44.32%. The survival rates after culturing in 0.3% and 1.5% bile salts for 17 h were 99.67% and 96.79%, respectively, and 88.76% and 71.34% after culturing in pH 3.0 and pH 2.0 for 17 h, respectively. After establishing the high-fat hamster model through a high-fat diet, hamsters were treated with different doses of probiotics. Regardless of dosage, triglyceride levels in the group fed with Lacticaseibacillus rhamnosus HCS01-013 was significantly lower than that of the model control group fed with normal saline. Lacticaseibacillus rhamnosus HCS01-013 has definite lipid lowering function and strong resistance to adverse environments, which provides a theoretical basis for related research for this strain and product development.
