Antialcoholism Effects of Curcuma Botanical Beverage in KM Mice
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Abstract:
The antialcoholism effect and possible mechanism of curcuma botanical beverage in KM mice were investigated in this paper. After establishing the model of high concentration alcohol-induced intoxication in mice, the anti-alcoholism effect of curcuma botanical beverage were evaluated according to the behavior of anti-inebriation test in mice, the concentration of ethanol in blood, the content or activity of the key enzyme of ethanol metabolism in vivo, and the changes of gastrointestinal tissue. The results showed that the latency of drunkenness was 235.00 min, which was significantly longer than that in the model group (p<0.05). The time required to regain consciousness was 232.00 min, which was significantly shorter than that in the model group (p<0.05). The concentration of ethanol in the blood was 4.21 mg/mL, which was significantly lower than that of the model group (p<0.01). The activities of alcohol dehydrogenase and acetaldehyde dehydrogenase were 3.74 U/mg prot and 8.36 U/mg prot, respectively, which were significantly higher than those of the model group (p<0.05). The contents of coenzyme I and reduced coenzyme I were 0.08 nmol/mg prot and 0.39 U/mg prot, respectively. The ratio of NADH to NAD+ was significantly higher than that of model group (p<0.01). The contents of cytochrome P450 and glutathione peroxidase were 78.51 pg/mg and 1341.00 pg/mg, respectively, which were significantly higher than those in the model group (p<0.01). The results of stomach and intestinal tissue observation and pathological section showed that low and high dosage of curcuma botanical beverage could alleviate the injury of intestinal tract caused by ethanol and reduce the intestinal bleeding and edema. The above results indicated that curcuma botanical beverage has obvious anti-alcoholism effect on drunken mice. The mechanism may be related to enhancing the activities of key enzymes and antioxidant enzymes in the pathway of ethanol metabolism, speeding up the metabolism of ethanol in vivo, and protecting the liver and gastrointestinal tract.
