Crude Momordica Grosvenorii Extract Inhibites H2O2-induced ROS Production and Apoptosis in MIN6 Cells
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The study is to investigate the protective effect and mechanism of crude Momordica grosvenorii extract (MGE) against hydrogen peroxide (H2O2)-induced apoptosis and oxidative damage in MIN6 cells. An oxidative damage cell model was established by the induction by H2O2 at different concentrations, and the optimal induction concentration was selected according to cell viability. The cell viability was determined by the MTT assay. Flow cytometry was used to detect the level of reactive oxygen species (ROS) and apoptosis rates of cells. The apoptosis-related factors were detected by Western blot. The results showed that the crude MGE (0~200 μg/mL) showed little effect on the viability of MIN6 cells, thus, 200 μg/mL of the crude Momordica grosvenorii extract was chosen for subsequent experiments. The crude MGE at 200 μg/mL significantly reversed the decrease (p<0.05) in H2O2-induced cell viability. The results of flow cytometry showed that compared with the treatment with H2O2 alone, the crude MGE decreased significantly (p<0.05) the H2O2-induced increase in ROS level, and inhibited H2O2-induced apoptosis of MIN6 cells. Moreover, the Western blot results revealed that the crude MGE could partially reversed H2O2-induced decrease in the expression of PCNA and increase in the expression of Bax (p<0.05). In summary, the crude MGE could inhibit H2O2-induced ROS generation and apoptosis in MIN6 cells. The results of this study can provide an experimental basis for the development of Momordica grosvenorii into functional foods for the prevention and treatment of diabetes.
