Quick Screening and Simultaneous Determination of 50 Doping Residues in Livestock by Ultra Performance Liquid Chromatography Coupled with Quadrupole-time of Flight Mass Spectrometry
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Abstract:
A method for quick screening of 50 illegal drugs in livestock by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) had been established. Livestock products were first hydrolyzed by β-glucuronidase/arylsulfatase, then extracted with ethyl acetate-methyl tert-butyl ether, purified by HLB, and determined by UPLC-Q-TOF/MS in positive ion mode. A high-resolution database was established for quick screening and accurate characterization. Precise quantification by isotope internal standard method can be achieved. The correlation coefficient of the calibration curves of each illegal drugs standards exceeded 0.99 in the range of 2~200 μg/L. The detection limit of various drugs could reach 0.1~0.5 μg/kg, and the limit of quantification was 0.3~1.5 μg/kg. The average recovery at three different concentration levels varied from 81.74% to 114.22%, and the relative standard deviation was 1.41%~8.91%. The established database realizes the quick screening and analysis of 50 illegal drugs without the need for standard products. This method is suitable for quick screening and simultaneous determination of low-concentration residues in livestock products.
