Isolation and Identification of Dominant Spoilage Molds from Preserves
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Abstract:
Mold contamination in the processing and storage of preserves directly affects the quality of preserves. In order to clarify the types of predominant spoilage molds in preserved preserves, this study isolated and purified spoilage molds in raisins, plums and apricots that were moldy after storage at room temperature for 4 months, and obtained 7 typical spoilage molds. The isolated spoilage molds were returned to the surface of raisins, plums and apricots for infection test verification. Four strains were identified as dominant spoilage molds based on the moldy time of preserves. Through the observation of the morphological characteristics of the colony, the microstructure of hyphae and conidium, combined with the sequence analysis of ribosomal transcribed spacer (rDNA its), the phylogeny was constructed and the dominant mold species were identified. The results showed that Cladosporium velox and Penicillium citreonigrum were the dominant molds in raisins, Alternaria tenuissima was the dominant spoilage molds of plum, and Penicillium sclerotiorum was the dominant spoilage molds of apricot. It provided a reliable method for the isolation and identification of spoilage mold in preserves, and laid a basis for the selection of subsequent sterilization methods and the optimization of storage conditions.
