Simultaneous Determination of Three Pesticides and Their Metabolite Residues in Litchi and Longan by High Performance Liquid Chromatography-Tandem Mass Spectrometry with QuEChERS
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Abstract:
An analytical method was established for the determination of difenoconazole, chlorantraniliprole, cyantraniliprole and their metabolite J9Z38 residues in litchi and longan by QuEChERS pretreatment method with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technique. Residues in litchi and longan samples were extracted with acetonitrile, purified with 50 mg C18, 50 mg PSA, and 10 mg Nano-ZrO2, and then detected by HPLC-MS/MS with separation column of 75 mm InfinityLab Poroshell 120 SB-C18, and 0.1% formic acid-acetonitrile as mobile phase with gradient elution under multi-reactive ion monitoring (MRM) and quantified by external matrix-matched standard. Good linearities for the four analytes in the range of 1~100 μg/kg were obtained and the correlation coefficients (r2) were from 0.9896 to 0.9999. The limits of quantitation (LOQs) were among 0.10 to 10 μg/kg. The recoveries in the litchi and longan were ranged from 82.48% to 99.17%, the intra-day precisions and inter-day precisions were 4.11%~7.77% and 6.10%~13.27%, respectively. The developed method have several advantages: easy to operate, simple and rapid, and high sensitivity. The method can be used for monitoring of difenoconazole, chlorantraniliprole, cyantraniliprole and their metabolite J9Z38 in real litchi and longan samples.
