Extraction of Total Flavonoids from Sophora japonica L. by Acidic Oxidized Electrolyzed Water
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Abstract:
In order to explore the pretreatment effect of acidic electrolyzed oxidizing water (AEOW) on the extraction of total flavonoids from plants, Sophora japonica was selected as experimental material and extracted by two-step method of AEOW pretreatment and ethanol reflux. Based on the results of single-factor experiment, the Box-Behnken method was used to optimize the extraction process of total flavonoids from Sophora japonica. The antioxidant activity in vitro was evaluated by measuring the ability of scavenging DPPH and hydroxyl free radicals. The results have confirmed that the pretreatment process of AEOW can obviously improve the yield of total flavonoids, because AEOW contains a certain amount of chlorine in oxidation state. When the available chlorine content (ACC) increased from 13.61 to 322.22 mg/L, the flavonoid yield increased from 7.16% to 20.88%. The optimum extraction process of total flavonoids from Sophora japonica was as follows: the ACC of AEOW was 250 mg/L, the pretreatment time was 160.53 min, the volume fraction of ethanol was 60.89%, and the ratio of liquid-to-solid was 36.60: 1 mL/g. The yield of total flavonoids from Sophora japonica was 19.67%. The antioxidant activities in vitro showed that the highest scavenging rates of total flavonoids from Sophora japonica on DPPH and hydroxyl free radical can reach 96.38% and 92.30%, respectively, indicating that it has good antioxidant activity and can be used as a natural antioxidant. In this research, AEOW was applied to the pretreatment of plant extraction for the first time, and a two-step extraction process of AEOW pretreatment and ethanol reflux was proposed, which provided experimental support and theoretical analysis for the extraction of total flavonoids from plants.
