Regulation of Hydrogen Peroxide on the Lignification of Chinese Flowering Cabbage after Harvest
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Abstract:
The effect of hydrogen peroxide on the storage and preservation of Chinese flowering cabbage were investigated by soaking in 5 mmol/L hydrogen peroxide and stored at 4 ℃. The changes of lignin content, activity of enzymes and relative expression levels of genes that involved in lignin biosynthesis in stem were carried out to explore the influence of H2O2 signal on the regulation of lignin synthesis of Chinese flowering cabbage. The results showed that respiration rate, lignin content, and endogenous H2O2 content of Chinese flowering cabbage were promoted by the hydrogen peroxide treatment. When stored for 12 days, the stem lignin content increased by 35.78% and H2O2 content increased by 2.71 times. Meanwhile, the activity of enzymes involved in lignin biosynthesis were accelerated by H2O2 treatment i.e., the activity of phenylalanine ammonia-lyase (PAL) and cinnamyl alcohol dehydrogenas (CAD) was increased by 85.70% and 15.53%, respectively, after storage for 9 days. The relative gene expression levels of lignin biosynthesis gene and involved in reactive oxygen generating, SOD1, SOD2, SOD3, and CAT3 involved in reactive oxygen scavenging were upregulated by H2O2 treatment during storage. These results indicated that the lignification of stalk in Chinese flowering cabbage was promoted by 5mM H2O2 treatment. It is concluded that H2O2 may alter the endogenous H2O2 content by affecting the homeostasis of ROS system, thereby, regulating the biosynthesis of lignin to affect the stalk lignification.
