Effect of Alcohol Extract of Xinjiang Ramulus Rose on Fatty Acid Induced Lipid Accumulation in HepG2 Cells
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Abstract:
To explore the influence of the alcohol extract of Xinjiang Branchlets Rosa rugosa Thunb. on the accumulation of fat in HepG2 cells, the alcohol extract of Branchlets Rosa rugosa Thunb. was used as the object, HepG2 cells were induced with free fatty acids (FFAs) to establish a fatty acid load model in vitro. The safe concentration of Branchlets Rosa rugosa Thunb. in the HepG2 cells was determined by MTT method. The triglyceride (TG) kit was used to measure the TG content of each group of cells after the intervention of the alcohol extract of Branchlets Rosa rugosa Thunb. Intracellular lipid droplet accumulation and lipid content of each group were determined by oil red O staining. Western blotting method was used to detect the protein levels of PPARα, ACOX1, and CPT1A in each group. The results showed that the safe concentration of alcohol extract of Branchlets Rosa rugosa Thunb. in HepG2 cells was equal to or less than 200 μmol/L. The fatty acid load model of HepG2 cells in vitro could be successfully established with FFAs concentration of 1.5 mmol/L for 24 h. Compared with the model group, the ethanol extract of Branchlets Rosa rugosa Thunb. markedly decreased the intracellular TG and lipid content induced by FFAs in a dose-dependent manner (p<0.05). The TG level decreased by 47.17%~60.38%, and the lipid content decreased by 25.00%~36.61%. Compared with the model group, the ethanol extract of Branchlets Rosa rugosa Thunb. could obviously up-regulate PPARα, ACOX1and CPT1A protein expression (p<0.05). The ethanol extract of Branchlets Rosa rugosa Thunb. can improve FFAs-induced lipid accumulation in HepG2 cells, and its anti-lipidemia effect isachieved by activating PPARα-mediated fatty acid oxidation metabolism pathway, and then reducing intracellular TG and lipid content.
