Evaluation of the Hypoglycemic Effects of Cyclocarya paliurus Based on a Zebrafish Biological Model
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Abstract:
In this study, 5 dpf zebrafish was used to investigate systematically zebrafish’s tolerance to glucose (GLU) and alloxan (ALX). Then, the feasibility and effectiveness of using the combination of GLU and ALX to establish a biological model of diabetic zebrafish were evaluated. On this basis, the tolerance of zebrafish to Cyclocarya paliurus (CP) was examined, and the evaluation of the hypoglycemic effect of CP was conducted. The results showed that the induction with the combination of 10 mg/mL GLU and 3.60 or 7.20 μg/mL ALX for 24 h could significantly increase the GLU levels by 51.52% (p<0.05) and 68.32% (p<0.05), respectively. The treatments with 200, 400 and 800 μg/mL acarbose decreased the GLU levels of zebrafish by 27.28% (p<0.05), 33.69% (p<0.05) and 38.75% (p<0.05), respectively, suggesting that the 24 h treatment with the combination of 10 mg/mL GLU and 3.60 μg/mL ALX could effectively establish diabetic zebrafish model. In addition, the research revealed that 400 and 800 μg/mL CP treatments could significantly decrease the GLU levels of 10 mg/mL GLU solution-treated zebrafish by 37.59% (p<0.05) and 38.57% (p<0.05), respectively. The treatments with 200, 400 and 800 μg/mL CP could significantly decrease the GLU levels by 27.43%, 32.75% (p<0.05) and 29.20% (p<0.05), respectively, in diabetic zebrafish. The results showed CP had obvious hypoglycemic effect, based on the evaluation of zebrafish with normal physiology and pathogenetic zebrafish. CP has a broad application prospect in the development of auxiliary hypoglycemic functional drinks.
