Isolation and Identification of the Pathogen Associated with Acute Hepatopancreatic Necrosis Disease in Imported Prawns and the Establishment of A Loop-mediated Isothermal Amplification (LAMP) Assay
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
In this study, 453 batches of samples were collected from prawns imported from Vietnam, Thailand and Malaysia during 2017~2018, to isolate and identify the pathogens of acute hepatopancreatic necrosis disease (AHPND). The analysis of the obtained results showed that the detection rate of the pathogen isolated from the hepatopancreas tissues of prawns was only 2.87%, whilst the detection rate reached 16.11% after enrichment culture. The prawn samples imported from Vietnam had the highest positive detection rate, with the positive detection rate of 312 batches of samples being 21.79%, and 11 positive strains were isolated with the rate of separation success being 15.07%. In order to identify quickly, sensitively and reliably the AHPND pathogens, a loop-mediated isothermal amplification (LAMP) assay was established for rapid detection of AHPND pathogens in this research. The results showed that only the AHPND pathogen had obvious amplification curve and color change, and the limit of plasmid detection was 1 fg/μL, which was 100 times higher than that of the conventional PCR method, and had high stability. The detection of 37 actual samples showed that the positive rate was 35.13%, which was higher than that of conventional PCR test. The LAMP rapid detection method established in this study is suitable for the specific detection of AHPND pathogens, and the isolation, identification and analysis of pathogens can also provide reference data for laboratory detection of imported prawns.
