Analysis and Comparison of Peanut Allergen Proteins Ara h2, Ara h3 and Sesame Protein 2S albumin in Pure Sesame Paste
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Abstract:
In this study, real-time PCR（RT-PCR）and enzyme-linked immunosorbent assay (ELISA) were used to detect peanut allergenic proteins Ara h2, Ara h3 and sesame protein 2S albumin in pure sesame paste. The specificity and reproducibility of the two methods were shown to be good. After verification, real-time PCR was used to detect the sesame protein 2S Albumin gene and peanut allergen Ara h2 gene, and ELISA was used to detect the peanut allergen Ara h3 protein. The 90 batches of samples detected by real-time PCR test contained sesame components (with CT value ranging from 22.00 to 33.80), and among which 45 batches of samples contained peanut components (CT value: 25.60~38.60). Besides these 45 batches s, ELISA also allowed the detection of peanut components in another 38 batches. By comparing and analyzing the results of the two methods and through sensitivity analysis research, it was found that the ELISA method had a lower detection limit. Although the results of the two methods were different, they were not contradictory, with both suitable for the detection of peanut sources in pure sesame samples. The real-time PCR method is appropriate for the samples with a high peanut content, whilst the ELISA method can be selected for samples with a low peanut content or unpredictable samples. At present, there are no relevant standards for the detection of allergens in sesame paste. This study provides reliable data for basic research, and is the results suggest that the regulatory authorities should establish relevant methods as soon as possible to supervise market behavior and ensure people's dietary safety.
