In Vitro Antioxidant Activity of the Metabolites of Walnut Endophytic Fungus LTS-6-6 and Their Effects on Oil Oxidation Stability
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
Taking the walnut endophytic fungus LTS-6-6 as the research object, the in vitro antioxidant activities of different-polarity extracts of its metabolites were determined by the ABTS free radical scavenging method, the hydroxyl free radical scavenging method and the Fe3+ reduction ability assay; The Schaal oven method was used to determine the effect of adding highly active extracts on the oxidation stability of rapeseed oil using the peroxide value and acid value of oil as indicators. The research results showed that among the four extracts, ethyl acetate extract (EAE) showed a stronger antioxidant activity, and exhibited strong scavenging abilities for ABTS radical and hydroxyl radical (scavenging rate ≥ 80%). The clearance rate of EAE was higher (by 71.39%) than that of petroleum ether extract (PEE). The contents of total phenols and flavonoids in EAE were 643.71 mg gallic acid/g and 102.05 mg rutin/g. The peroxide value and acid value of the rapeseed oil treated with 0.02% EAE and stored at 70 ℃ for 8 days were 5.88 mmol/kg and 0.85 mg/g, respectively. As predicted by the Arrhenius formula, the rapeseed oil with 0.02% EAE had a storage period of 256 days at 20 ℃, which was 192 days longer than that of the blank group, indicating that the addition of EAE effectively inhibited the oxidative rancidity of rapeseed oil.
