Tea Seed Saponin Ameliorated Lipid Metabolism and Oxidative Stress in High Fat Diet Fed Mice
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Abstract:
In this study, the effect of tea seed saponin on lipid metabolism and oxidative stress caused by high-fat diet in mice was investigated. The food intake and body weight changes of mice were observed. The levels of serum superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), total cholesterol (TC), triglycerides (TG), low density lipoprotein (LDL-C) and high density lipoprotein (HDL-C) were determined by relevant commercial kits. The hepatic steatosis levels were observed by H&E staining assay. The hepatic protein levels of AMPK, p-AMPK, SIRT1, PGC-1α and PPAR-γ were detected by western blot assay. The experimental results showed that TSS treatment significantly reduced the body weight, and organ index of epididymal fat, perirenal fat and abdominal fat in HFD mice (p<0.05). The serum levels of TC (37.83%), TG (64.77%) and LDL-C (76.83%) were reduced in high dose of TSS treated mice than that in HFD mice. TSS treatment significantly reduced the hepatic steatosis and promoted the protein levels of p-AMPK (3.92 times, 4.95 times and 6.63 times), SIRT1 (0.86 times, 1.36 times and 1.77 times), PGC-1α (3.10 times, 3.11 times and 4.33 times) and PPAR-γ (2.83 times, 4.27 time and 5.51 times) in HFD mice (p<0.05). After treated by TSS, serum levels of SOD and GSH were increased in HFD mice. In addition, serum MDA was also decreased in TSS treated HFD mice. Taken together, our results suggested that TSS reduced fat accumulation, improved the lipid metabolic disorder and attenuated the oxidative stress status in mice induced by HFD.
