In Vitro Antioxidant Activity of Fucoidan-containing Sulfated Polysaccharide and Its Prolongation of the Lifespan of Caenorhabditis elegans
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Abstract:
The in vitro antioxidant effect of fucoidan-containing polysaccharide (FSP) was investigated. Taking Caenorhabditis elegans (C. elegans) as the model animal, an acute oxidative stress model was established using the oxidizing agent, H2O2. The effects of FSP on lifespan, mobility, age pigment and oxidative stress indices were determined. The research showed that FSP exhibited an ability to scavenge hydroxyl radicals and this effect was in dose-dependent; FSP (50, 100 and 200 μg/mL) prolonged the lifespan of C. elegans in a dose-dependent manner (by 7.24%, 26.70% and 48.42%, respectively); FSP significantly increased the motility of nematodes, with the motility of the 200 μg/mL FSP treatment group reaching 73.50%, and the accumulation of lipofuscin and the degree of apoptosis decreased; Under 0.1% H2O2-induced oxidative stress, the treatment with 200 μg/mL FSP enhanced the antioxidant capacity through increasing the activities of antioxidant enzymes, SOD and CAT; The qRT-PCR results showed that FSP influenced the lifespan of nematodes through regulating the expression of related genes in the Insulin/IGF-1 signaling pathway. In summary, FSP could exert protective effects on oxidative damage in nematodes through extending the lifespan of C. elegans, enhancing its mobility, promoting the activities of antioxidant enzymes, regulating the expression of key genes and inhibiting the level of oxidative stress.
