Rapid Determination of Malachite Green and Crystal Violet Residues in Aquatic Products by Raman Spectroscopy
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Abstract:
In order to realize the rapid detection of malachite green and crystal violet residues in aquatic products, a rapid surface enhanced Raman spectroscopy method (SERS) for malachite green and crystal violet residues in aquatic products was established. By means of acetonitrile extraction, petroleum ether purification, potassium borohydride reduction and DDQ oxidation, the amount of colloidal gold, extract and coagulant was determined. The Raman characteristic displacement of malachite green was 795, 939, 1215, 1610 cm-1. The Raman displacement of crystal violet was 795, 939, 1610 cm-1. According to its Raman spectrum characteristic peak and its intensity, the malachite green and crystal violet were determined qualitatively and rapidly. Combined with the detection probability model (POD), the detection limit of malachite green and crystal violet shrimp and crab was determined to be 1.00 μg/kg, the detection limit of fish and shellfish is 0.50 μg/kg, sensitivity ≥95%, specific ≥95%, false negative ≤5%, false positive ≤5%, which meets the qualitative requirements of rapid testing. The method is simple, rapid and accurate. It only takes 40 minutes from sample pretreatment to result display. It can be used for the on-site rapid detection of malachite green and crystal violet in aquatic products.
