Synergistic Inhibitory Activity of Momordica charantia Saponin and Whey Protein Hydrolysate on Dipeptidyl Peptidase-IV
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Abstract:
To evaluate the inhibitory activity of dipeptidyl peptidase-IV (DPP-IV) from natural animal products, Momordica saponins (MS), whey protein pepsin hydrolysates (WPPHs), and whey protein trypsin hydrolysates (WPTHs) were used as raw materials, and the hydrolysis conditions were optimized by single factor test and response surface analysis, and then MS and WPPHs and WPTH were compounded, combined with simulated in vitro digestion, the inhibitory activity of DPP-IV was explored. The results showed that MS, WPPHs and WPTHs had DPP-IV inhibitory activity. The optimal hydrolysis conditions were as follows: When the enzyme was added at 4% and the pH was 2.7 for 2 h, the highest DPP-IV inhibitory rate of WPPHs was 15.43%; The highest DPP-IV inhibition rate of WPTHs was 14.62% when the addition amount was 4% and pH 7.6 was enzymatically hydrolyzed for 4 h. MS and whey protein hydrolysate had a synergistic effect on inhibitory activity of DPP-IV. When the combined volume ratio of MS, WPPHs and WPTHs is 1:1, it is significantly higher than that of their accumulation alone (p<0.05). After gastric digestion, the DPP-IV inhibition rate of WPPHs decreased by 2.02%, and the DPP-IV inhibition rate of MS and WPTHs increased by 0.99% and 7.01%, respectively; after intestinal digestion, the DPP-IV inhibition rate of WPPHs increased by 2.23%, while the DPP-IV inhibition rate of MS and WPTHs decreased by 4.12% and 2.70%, respectively. The results provide basic data for the synergistic inhibitory activity of natural animal and plant products against dipeptidyl peptidase-IV, and provide a new direction for the development of hypoglycemic functional products.
