Isolation and Identification of a Basophilic Bacterium BD from “Bursted” Pidan and De novo Sequencing Analysis of the Strain
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Abstract:
A Gram-negative bacterial strain named BD was isolated from the “bursted” Pidan by traditional microbial culture method. The strain was identified by morphological observation, biochemical analysis and 16S rDNA. The phylogenetic tree was also constructed. The growth performance, alkali resistance and the gas production capacity of the strain were investigated through bacteriological experiment. The whole genome of the strain was sequenced using De novo system and a draft map was obtained. The results showed that the physiological and biochemical characteristics of the strain were Vibrio genus, and the homology of 16S rDNA sequence with Vibrio metschnikovii reached 99.92%. This strain could grow in a high alkali (pH 12) environment, and the optimal growth temperature was 25~40 ℃. De nove sequencing of the strain showed that the genome size of the strain was 3.70 Mb, encoding 3381 genes in total. Functional annotation of protein-coding genes showed that the genome of this strain contained a large number of genes related to carbohydrate metabolism pathway and membrane transport function pathway. This study provides a theoretical basis for the development of the "burst" control scheme of Pidan processing, and provides a reference for the mining of functional genes related to the adaptation of microorganisms to high alkali and high lipid environment.
