The Ameliorating Effect of Brassica napiformis Superfine Powder on Chronic Obstructive Pulmonary Disease in Mice
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Abstract:
The ameliorating effect of Brassica napiformis superfine powder (BUP) on the mice with chronic obstructive pulmonary disease (COPD) induced by intranasal instillation of lipopolysaccharide was investigated. The results showed that the active ingredients of BUP, such as soluble dietary fiber, polyphenols, flavonoids, glucosinolates and isothiocyanates, had higher dissolution rates (which are increased by 77.27%, 63.89%, 17.86%, 26.72% and 42.86% respectively) compared with the Brassica napiformis powder (BOP) that was prepared via crushing and passing through a 60-mesh sieve; BUP could ameliorate weight loss, emphysema and pulmonary fibrosis in COPD mice, increase the proportion of regulatory T cells in broncho alveolar lavage fluid (BALF) of COPD mice, reduced the proportion of macrophages, dendritic cells and neutrophils, and down-regulate the levels of IL-1β and IL-6 while up-regulating IL-10 level in the lung tissues of COPD mice. BUP could prevent from p65 entering the nucleus to promote the expression of inflammatory factors through inhibiting the phosphorylation of IκB and NF-κB p65 in lung tissues. At the same time, BUP could balance the level of reactive oxygen species in the BALF of COPD mice, improve the levels of oxidative stress-related factors, such as nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), and promote the expressions of Nrf2, heme oxygenase-1 (HO-1) and quinone oxidoreductase-1 (NQO-1) in the lung tissues of COPD mice. The research results showed that the ameliorating effect of BUP on COPD mice was greater than that of BOP, due to the higher dissolution rate of BUP’s active ingredients, and the underlying mechanism might be related to its anti-inflammatory and antioxidant effects. The experimental results provide a new reference for the development and utilization of Brassica napiformis.
