Effects of Pleurotus citrinipileatus Polysaccharides on the of Secretion Cytokines and Nitric Oxide Synthase (NOS) in An in Vitro Induced Inflammation Model
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Abstract:
Pleurotus citrinipileatus was used as the raw material, and crude polysaccharides were extracted by water extraction and alcohol precipitation from its fruiting bodies of. The Sevage method was used to remove proteins, and two components, PSI and PSII were obtained after purification by DEAE-Cellulose ion exchange and Superdex-75 gel chromatography. The in vitro inflammation model was established by lipopolysaccharide (LPS) stimulation. The optimal doses of the two polysaccharides were screened by the MTT method. The activity of nitric oxide synthase (NOS) was determined by the absorbance assay, and the levels of secreted cytokines, IL-1β, IL-6 and TNF-α, were determined by enzyme linked immunosorbent assay kits to evaluate the anti-inflammatory effect of the polysaccharides on RAW264.7 cells. The results showed that the cellular inflammation level reached the highest when the concentration of LPS was 5 μg/mL. On the basis of the cell model, the optimal dose of PSI and PSII were 500 μg/mL and 200 μg/mL, respectively. Compared with the LPS model group, the inhibition rates of PSI on NOS activity, IL-1β, IL-6 and TNF-α were 37.54%, 28.84%, 28.27% and 30.25%, respectively. The inhibitory rates of PSII on NOS activity, IL-1β, IL-6 and TNF-α were 51.24%, 37.56%, 31.35 % and 32.67%, respectively. The research confirmed that PSI and PSII had the alleviating effects on LPS-induced inflammatory cells, which were mainly manifested in inhibiting the level of secreted inflammatory cytokines, promoting cell proliferation, and reducing the phagocytic ability of macrophages. Compared with PSI, PSII had a greater inhibitory effect on the secretion levels of nitric oxide synthase (NOS) and cytokines, IL-1β, IL-6 and TNF-α, thereby exhibiting a better anti-inflammatory effect. Key words:
