IQ-Check Salmonella II Kit-based Method for the Detection of Salmonella in Foods
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Abstract:
The IQ-Check Salmonella II kit-based method was evaluated through the detection of standard strains and artificially contaminated Salmonella in foods. In this study, the kit method was used to detect 50 Salmonella strains with different serotypes and 50 non-Salmonella strains, and the sensitivity and specificity of this method were analyzed. By detecting the artificially contaminated Salmonella in food samples (including liquid milk, infant formula milk powder, meat and meat products), the consistency of the kit method and the GB 4789.4-2016 method was evaluated. The experimental results showed that when the concentration of Salmonella was 103 CFU/mL or above, all 50 strains of Salmonella could be detected by the kit method, and the average detection limit of Salmonella with different serotypes was 6.98×102 CFU/mL. The results of the kit method for 50 non-Salmonella strains were all negative, indicating that the kit method showed good specificity. The positive detection rates of the kit method and the GB method for the artificially contaminated Salmonella in food samples were 98.77% (161/163) and 96.32% (157/163), respectively. The relative accuracy was 96%, 99%, 97%, and the overall accuracy was 97.33%; the relative sensitivity was 96.22%, 100%, 100%, and the overall sensitivity was 98.73%.The relative specificity was 95.74%, 98.15%, 92.86%, and the overall specificity was 95.80%. The consistency analysis was carried out according to ISO 16140, and the results showed that there was no statistically significant difference between the two methods. The results showed that the kit method had the characteristics of high sensitivity and strong specificity, and was highly consistent with the GB method in the detection of artificially contaminated food samples. The kit method is worthy of popularization and application in the rapid detection of Salmonella in food.
