Analysis and Identification of Main Chemical Components in Ethanol Extract of Ramie Seeds Based on UPLC-Orbitrap-HRMS Technology
Article
Figures
Metrics
Preview PDF
Reference
Related
Cited by
Materials
Abstract:
The main chemical composition of the ethanol extract from ramie seeds were analyzed and identified using ultra performance liquid chcromatography-orbitrap-high resolution mass spectrometer (UPLC-Orbitrap-HRMS). UPLC conditions: Agilent 5TC-C18 chromatographic column (4.6 mm×250 mm, 5 μm), the mobile phase gradient elution with 0.2% formic acid water solution and acetonitrile. HRMS conditions: electrospray ion source (ESI), ion trap mass analyzer (orbitrap), spray voltage 3500 V (positive ion mode). The mass spectrum information was analyzed by mass spectrometry data of standards, MsBank mzCloud mass spectrometry databases and relevant literature. Twenty compounds were identified from the ethanol extract of ramie seeds. In which, there are ten flavonoids, including proanthocyanidin dimer, rutin, isoquercitrin/hyperioside, kaempferol-3-O-β-D-rutin, quercetin-3-O-β-D-xyloside, kaempferol-3-O-β-D- glucoside/kaempferol-3-O-β-D-galactoside, querceitrin, quercetin, kaempferol and afzelin. The flavonoids in the ethanol extract of ramie seeds are mainly flavonoid aglycones with antioxidant activity, indicating that ramie seeds have potential antioxidant activity. This study provides a scientific basis for the development and utilization of ramie seed.
